Studying the immune molecular mechanism of clams in different states is helpful for analysis of fundamental differences between the susceptible and resistant groups. philippinarum have yet to be fully elucidated. However, the molecular mechanisms underlying different tolerances and levels of resistance to V.
Transcriptome analyses of responses and defense against different pathogen-associated molecular patterns in R. anguillarum infection to determine the molecular classification and evolutionary model in invertebrates and the basis of the innate immune response of the TLR signaling pathway ( 16). More recently, a transcriptome study analyzed the toll-like receptor (TLR) family in R. anguillarum infection revealed simple sequence repeats and single nucleotide polymorphisms in response to infection ( 13). Several studies using transcriptome sequencing techniques have explored the use of shellfish for disease resistance immunity ( 14, 15). anguillarum, is an important bacterial infection in Manila clam, which may lead to the death of farmed clams and causing economic losses ( 13). Vibriosis, a hemorrhagic septicemic disease caused by the bacterium V. philippinarum has faced tremendous challenges caused by bacterial disease, protistan parasites, and environmental stressors ( 10– 12). It has many advantages as an aquaculture species, including wide salinity and temperature resistance, rapid growth, and pollution tolerance ( 9). Ruditapes philippinarum, a traditionally commercial clam with high nutritive value and delicate flavor, is widely distributed along the coasts of China, Japan, and Korea ( 8). Comparative analysis of the immune responses of Crassostrea gigas under challenge from different Vibrio strains and conditions demonstrated genes could be used as immune responsive biomarkers to monitor early changes in oysters in response to bacterial infection ( 7). farreri, it was reported that the tumor suppressor QM gene can protect against challenge with pathogens such as V. galloprovincialis using proteomics and metabolomics ( 5). anguillarum were investigated in the hepatopancreas of M. constricta demonstrated the important role of galectins as pattern recognition receptors in innate immunity, with results suggesting that ScGal2 has an indispensable role in the recognition of Gram-negative bacteria in this species ( 4). Mollusks lack adaptive immunity and are completely dependent on innate immunity to resist invasion by potentially harmful microorganisms. anguillarum infection in mollusks, such as Sinonovacula constricta, Mytilus galloprovincialis, and Chlamys farreri ( 4– 6). Various studies have focused on immune responses and molecular characteristics of V. Vibrio anguillarum is a marine pathogen that can cause fatal hemorrhagic sepsis (vibriosis) in farmed and wild fish, as well as mollusks and crustaceans ( 1– 3). The results obtained here provide a reference for future immunological research focusing on the response of R. anguillarum increases our understanding of the resistant genes and key pathways related to Vibrio challenge in this species.
philippinarum resistant and nonresistant to V. anguillarum infection, which might indicate the role of lectin in the immune response to V. The lectin gene in the phagosome pathway was expressed at a significantly higher level after V. Ten genes were selected and verified by RT-qPCR, and nine of the gene expression results were consistent with those of RNA-seq. anguillarum infection, including C-type lectin domain, glutathione S-transferase 9, lysozyme, methyltransferase FkbM domain, heat shock 70 kDa protein, Ras-like GTP-binding protein RHO, C1q, F-box and BTB/POZ domain protein zf-C2H2. Host immune factors were identified that responded to V. philippinarum showing different levels of resistance to challenge with Vibrio anguillarum were constructed and RNA-seq was performed using the Illumina sequencing platform. In this study, the transcriptome libraries of R. philippinarum has been negatively impacted by various bacterial pathogens.
However, in recent years, the aquaculture of R. The clam Ruditapes philippinarum is an important species in the marine aquaculture industry in China.
0 kommentar(er)
